SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Di,Xiuzhen; Bai,Nan; Zhang,Xin; Liu,Bin; Ni,Wentao; Wang,Jin; Wang,Kai; Liang,Beibei; Liu,Youning; Wang,Rui.

The aim of this meta-analysis was to compare the efﬁcacy of metronidazole and vancomycin for the treatment of Clostridium difﬁcileinfection, especially to investigate which agent was superior for treating either mild or severe C. difﬁcileinfection. A meta-analysis of randomized controlled trials and cohort studies identiﬁed in Pubmed, Embase, and the Cochrane Library was conducted. Four randomized controlled trials and two cohort studies involving 1218 patients were included in this meta-analysis. Metronidazole was inferior to vancomycin for treating C. difﬁcileinfection in terms of both initial clinical cure rates (risk ratio, RR = 0.91, 95% conﬁdence interval, CI = 0.84-0.98, p= 0.02) and sustained cure rates (RR = 0.88, 95% CI = 0.82-0.96, p= 0.003)....

Amphotericin-B and vancomycin-loaded chitosan nanofiber for antifungal and antibacterial application

Palavras-chave: Clostridium difﬁcile; Clostridium difﬁcileinfection (CDI); Vancomycin; Metronidazole.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400339

BJPS

Karimi,Soroush; Moradipour,Pouran; Azandaryani,Abbas Hemati; Arkan,Elham.

In the present study, a mucoadhesive non-woven fiber mat (d= 116 nm) was fabricated by the electrospinning method using chitosan (80% Wt), polyethylene oxide (10% Wt), cysteine (4% Wt) and drugs (6% Wt), respectively. In addition, a comparative study was conducted to define effect of drugs and mucoadhesive agent on the nanofiber formation. FTIR, SEM, DSC and DMA were used to investigate the chemical and physical properties of the nanofibers. In vitro release of the drugs was assessed over a 48-hour period by the total immersion method. Release data were ﬁtted to kinetic models, including the zero-order, ﬁrst-order, Higuchi matrix, and Hixson-Crowell. Zone inhibition investigations were used to describe the inhibition content of vancomycin and amphotericin...

Clinical failure of vancomycin treatment of Staphylococcus aureus infection in a tertiary care hospital in southern Brazil

Palavras-chave: Mucoadhesive; Vancomycin; Amphotericin-B; Electrospinning; Aphthous; Chitosan.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100521

Cost-effectiveness of linezolid versus vancomycin in mechanical ventilation-associated nosocomial pneumonia caused by methicillin-resistant staphylococcus aureus

Lutz,Larissa; Machado,Adão; Kuplich,Nadia; Barth,Afonso Luís.

We describe a case of clinical failure of vancomycin treatment of Staphylococcus aureus infection and the laboratory characteristics of the organism in a tertiary referral university hospital in southern Brazil. An 11-month-old male patient presented with pneumonia and S. aureus was isolated from his respiratory tract. Initial treatment with oxacillin and gentamicin was ineffective. Vancomycin was added to the regimen as the patient worsened, but after the 30th day of vancomycin treatment S. aureus was isolated from the blood. This isolate had a minimum inhibitory concentration (MIC) for vancomycin of 4 µg/mL. After pre-incubation with vancomycin the isolate displayed an increase in the expression of vancomycin resistance and colonies grew in the presence...

Tipo: Info:eu-repo/semantics/report

Palavras-chave: S. aureus; Resistance; Vancomycin.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300008

Machado,Adão R. L.; Arns,Clovis da Cunha; Follador,Wilson; Guerra,Aline.

Linezolid, an oxazolidinone-class antimicrobial agent, is a new drug; its use has frequently been questioned due to its high price. However, recent trials have demonstrated that the use of linezolid in mechanical ventilation-associated nosocomial pneumonia caused by methicillin-resistant Staphylococcus aureus (VAP-MRSA) may be justified due to its improved efficacy compared to vancomycin. Price and cost have different magnitudes, and clinical efficacy should always be considered in the decision-making process. Our objective was to determine whether linezolid treatment was more cost-effective than vancomycin for treating VAP-MRSA. METHODOLOGY: Elaboration of an economic model from a metanalysis of previous clinical trials comparing both drugs, through a...

Daptomycin and vancomycin non-susceptible methicillin-resistant Staphylococcus aureus clonal lineages from bloodstream infection in a Brazilian teaching hospital

Palavras-chave: Linezolid; Vancomycin; Staphylococcus aureus; Pneumonia; Ventilator; Cost; Pharmacoeconomic.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000300001

Damasco,Andreia Paredes; Costa,Thaina Miranda da; Morgado,Priscylla Guimarães Migueres; Guimarães,Lorrayne Cardoso; Cavalcante,Fernanda Sampaio; Nouér,Simone Aranha; Santos,Kátia Regina Netto dos.

ABSTRACT Introduction: This study aimed to characterize Staphylococcus aureus isolates from bloodstream infections in patients attending a teaching hospital, between 2011 and 2015. Methods: The minimum inhibitory concentration for daptomycin, linezolid, oxacillin, teicoplanin, vancomycin, and trimethoprim/sulfamethoxazole was accessed by broth microdilution. SCCmec type and clonal profile were determined by molecular tests. Vancomycin heteroresistance was evaluated using screening tests and by population analysis profile/area under the curve. Results: Among 200 S. aureus isolates, 55 (27.5%) were MRSA, carrying SCCmec II (45.5%) or IV (54.5%). The most frequent MRSA lineages were USA100 (ST5-II) (45.5%) and USA800 (ST5-IV) (30.9%). Six isolates were...

Detection of Staphylococcus aureus with an intermediate profile to vancomycin (VISA) isolate from Santa Maria, RS

Palavras-chave: Bloodstream infection; S. aureus; Vancomycin; Daptomycin.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200139

Marques,Jaciane Baggiotto; Dalmolin,Tanise Vendruscolo; Bonez,Pauline Cordenonsi; Agertt,Vanessa Albertina; Campos,Marli Matiko Anraku de; Santos,Roberto Christ Vianna.

Twenty-three isolates of Staphylococcus aureus resistant to methicillin have been analyzed, being found a clinical isolate of VISA through microdilution technique. The others techniques were unable to detect such isolates. This is the first study that shows the presence of VISA in clinical isolates in the city of Santa Maria-RS.

Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth

Palavras-chave: Staphylococcus aureus; Vancomycin; Microdilution.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100040

Genet. Mol. Biol.

Souza,Fernanda Timm Seabra; Mello,Alexandre Silva de; Michelin,Kristiane; Coelho,Janice Carneiro.

The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and...

Emergence of VanB phenotype-vanA genotype in vancomycin-resistant enterococci in Brazilian hospital

Palavras-chave: Amikacin; Cephalothin; Clindamycin; Vancomycin; Fibroblasts; Cell culture.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300023

Zanella,Rosemeire Cobo; Lima,Marisa de Jesus Castro; Tegani,Luciana Soares; Hitomi,Adriana; Brandileone,Maria Cristina de Cunto; Palazzo,Izabel Cristina V.; Darini,Ana Lúcia da Costa.

In Brazil, vancomycin-resistant enterococci (VRE) have been reported as nosocomial pathogens since 1998. Recently, in a VRE surveillance in a hospital, we detected two Enterococcus faecalis isolates with vanA genotype and susceptible to teicoplanin. This is the first report of VanB phenotype-vanA genotype enterococci isolated from humans in Brazil.

Endothelial, renal and hepatic variables in wistar rats treated with Vancomycin

Palavras-chave: Enterococci; VanA genotype; VanB phenotype; Vancomycin; VRE.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200004

Anais da ABC (AABC)

BRUNIERA,FELIPE R.; FERREIRA,FELIPE M.; SAVIOLI,LUIZ R.M.; BACCI,MARCELO R.; FEDER,DAVID; PEREIRA,EDIMAR C.; PEDREIRA,MAVILDE L.G.; PETERLINI,MARIA A.S.; PERAZZO,FÁBIO F.; AZZALIS,LIGIA A.; ROSA,PAULO C.P.; JUNQUEIRA,VIRGINIA B.C.; SATO,MONICA A.; FONSECA,FERNANDO L.A..

Vancomycin (VCM) is indicated in combat against Gram-positive infections, but it is not considered a first-choice drug because of its adverse effects. It is believed that oxidative stress is the primary mechanism of endothelial injury and the consequent VCM toxicity, which varies from phlebitis to nephrotoxicity. Moreover, dose recommendations, dilution, rates and types of infusion are still controversial. The aim of this study was to determine the effect of different VCM dilutions in endothelial, liver and kidney injuries by biochemical parameters and histopathological analysis. Wistar rats were randomly divided into six groups and subjected to femoral vein cannulation for drug administration. Control groups received 0.9 ml of saline and the others...

Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil

Palavras-chave: Vancomycin; Acute kidney injury; Oxidative stress; Hepatotoxicity; Endothelial injury.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401963

BJMBR

Camargo,I.L.B.C.; Barth,A.L.; Pilger,K.; Seligman,B.G.S.; Machado,A.R.L.; Darini,A.L.C..

In 2000, Enterococcus faecalis resistant to vancomycin was first reported at a tertiary hospital in Porto Alegre, southern Brazil. The resistance spread to other hospitals and surveillance programs were established by hospital infection committees to prevent the spread of vancomycin-resistant enterococci. In February 2002, an isolate initially identified at the genus level as Enterococcus was obtained by surveillance culture (rectal swab) from a patient admitted to a hospital for treatment of septic arthritis in the shoulder. The isolate proved to be resistant to vancomycin by the disc diffusion method and confirmed by an E-test resulting in a minimal inhibitory concentration of > or = 256 µg/ml. This isolate was sent to a reference laboratory...

Glycopeptides susceptibility among enterococci isolated from a poultry farm in São Paulo, Brazil (1996/1997)

Palavras-chave: Enterococcus gallinarum; VanA phenotype; Vancomycin; Resistance.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100012

Leme,Ivani Lúcia; Ferreira,Antonio José Piantino; Bottino,José Américo; Pignatari,Antonio Carlos C..

Enterococci resistance to glycopeptides was evaluated em Brazilian poultry fed with feed supplemented with avoparcin as growth promoter. The susceptibility to the glycopeptides avoparcin, teicoplanin and vancomycin was determined for 217 enterococci isolated from cloacal swabs (one swab per bird) in tests and controls groups. Tests group comprised three groups (A, B and C) of Hubbard broiler-chickens 14, 21 and 35 days old, respectively. These birds were from one single farm, with a common feed source supplemented with avoparcin (10 mg/kg of feed). Controls groups (1 and 2) comprised 25 and 42 days old broilers, respectively, obtained from the Faculty of Veterinary Medicines aviary (University of São Paulo) where avoparcin was never used. No glycopeptide...

Healthcare-associated vancomycin resistant Enterococcus faecium infections in the Mansoura University Hospitals intensive care units, Egypt

Palavras-chave: Glycopeptides; Avoparcin; Vancomycin; Resistance; Enterococcus.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100013

Moemen,Dalia; Tawfeek,Doaa; Badawy,Wafaa.

Vancomycin resistant Enterococcus faecium (VREF) ia an emerging and challenging nosocomial pathogen. This study aimed to determine the prevalence, risk factors and clonal relationships between different VREF isolates in the intensive care units (ICUs) of the university hospitals in our geographic location. This prospective study was conducted from July, 2012 until September, 2013 on 781 patients who were admitted to the ICUs of the Mansoura University Hospitals (MUHs), and fulfilled the healthcare-associated infection (HAI) criteria. Susceptibility testing was determined using the disk diffusion method. The clonal relationships were evaluated with pulsed field gel electrophoresis (PFGE). Out of 52 E. faecium isolates, 12 (23.1%) were vancomycin resistant....

In vitro susceptibility of methicillin-resistant Staphylococcus aureus isolates from skin and soft tissue infections to vancomycin, daptomycin, linezolid and tedizolid

Palavras-chave: Enterococcus faecium; ICU; Vancomycin; Risk factors; PFGE.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300777

Múnera,Johanna Marcela Vanegas; Ríos,Ana María Ocampo; Urrego,Daniela Montoya; Jiménez Quiceno,Judy Natalia.

Abstract Introduction Treatment of multidrug-resistant Gram-positive infections caused by Staphylococcus aureus remains as a clinical challenge due to emergence of new resistance mechanisms. Tedizolid is a next-generation oxazolidinone, recently approved for skin and soft tissues infections. We conducted a study to determine in vitro susceptibility to vancomycin, daptomycin, linezolid and tedizolid in MRSA clinical isolates from adult patients with skin and soft tissue infections. Material and methods Methicillin-resistant S. aureus isolates were collected in three tertiary-care hospitals of Medellin, Colombia, from February 2008 to June 2010 as part of a previous study. Clinical characteristics were assessed by medical records and MIC values were...

In vitro susceptibility to methicillin, vancomycin and linezolid of staphylococci isolated from bloodstream infections in eastern Turkey

Palavras-chave: MRSA; Skin and soft tissues infections; Tedizolid; Linezolid; Vancomycin; Daptomycin.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500493

Tekin,Alicem; Dal,Tuba; Deveci,Özcan; Tekin,Recep; Özcan,Nida; Atmaca,Selahattin; Dayan,Saim.

Staphylococcus species are one of the major causes of bacterial bloodstream infections. Multi-resistant staphylococci infections are major therapeutic problems. This study was aimed to detect methicillin, linezolid and vancomycin susceptibilities of Staphylococcus isolates. A total of 870 Staphylococcus strains isolated from blood cultures of hospitalized patients with BSI. Antimicrobial susceptibilities of methicillin, linezolid and vancomycin were detected according to the Clinical and Laboratory Standards Institute (CLSI). A total of 771 (88.6%) isolates were coagulase-negative staphylococci (CoNS). 700 (80.5%) isolates were methicillin-resistant (MR) and 170 (19.5%) were methicillin-susceptible (MS). All the MS isolates were also susceptible to...

Influence of antibiotics on embryogenic tissue and Agrobacterium tumefaciens suppression in soybean genetic transformation

Palavras-chave: Staphylococcus; Methicillin; Linezolid; Vancomycin.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300010

Bragantia

Wiebke,Beatriz; Ferreira,Fabricio; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Droste,Annette.

The influence of different antibiotic treatments in soybean genetic transformation was evaluated. First, an assay was performed to verify how different antibiotic treatments affect soybean embryogenic tissues. The effect of carbenicillin at 500 mg L-1 was genotype-dependent. This antibiotic did not affect embryo survival of cv. IAS5, but a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control. On the other hand, cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars. Finally, the association of cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment. Thereafter, a second experiment was carried...

MRSA from Santa Catarina State, Southern Brazil: intriguing epidemiological differences compared to other Brazilian regions

Palavras-chave: Agrobacterium tumefaciens; Carbenicillin; Cefotaxime; Genetic transformation; Glycine max; Vancomycin.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052006000400002

Silveira,Alessandro C.O.; Cunha,Gabriela R.; Caierão,Juliana; Cordova,Caio M. de; d'Azevedo,Pedro A..

Methicillin-resistant Staphylococcus aureus(MRSA) is one of the most frequently isolated agents in both nosocomial and community settings. It is a constant challenge for antibacterial therapy. Therefore, it becomes essential to understand the epidemiology of MRSA isolates in the institution and/or region to guide empirical therapy. The objective of this study was to evaluate the epidemiological characteristics of MRSA isolates in the state of Santa Catarina, Brazil, and determine if there is a clonal spread. We evaluated 124 clinical isolates of MRSA obtained from various anatomical sites from patients in the state of Santa Catarina in Southern Brazil. The antimicrobial susceptibility proﬁle was evaluated by disk diffusion and minimum inhibitory...

Rapid detection of Vancomycin-Resistant Enterococci (VRE) in rectal samples from patients admitted to intensive care units

Palavras-chave: Antimicrobial resistance; Vancomycin; Oxacillin.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400384

d'Azevedo,Pedro Alves; Santiago,Kelly Aline de Souza; Furtado,Guilherme Henrique Campos; Xavier,Diego Batista; Pignatari,Antonio Carlos Campos; Titze-de-Almeida,Ricardo.

The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR for detection and identification of vanA and vanB resistance genes. The screening performance was analyzed in 114 specimens collected from four intensive care units. The swabs were collected at two periods: (1) during a VRE outbreak (February 2006, n=83 patients) and (2) at the post-outbreak period, after adoption of infection control measures (June 2006, n=31 patients). Forty-one/83 VRE (49.4%) and...

Serum levels of vancomycin: is there a prediction using doses in mg/kg/day or m2/day for neonates?

Palavras-chave: Enterococcus; VRE; Vancomycin; Diagnostic; PCR.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400010

Romanelli,Roberta Maia de Castro; Anchieta,Lêni Márcia; Fernandes,Juliana Chaves Abreu; Lima,Mariana Antunes Faria; Souza,Taís Marina de; Rosado,Viviane; Clemente,Wanessa Trindade; Camargos,Paulo Augusto Moreira.

Abstract Coagulase-negative Staphylococcus has been identified as the main nosocomial agent of neonatal late-onset sepsis. However, based on the pharmacokinetics and erratic distribution of vancomycin, recommended empirical dose is not ideal, due to the inappropriate serum levels that have been measured in neonates. The aim of this study was to evaluate serum levels of vancomycin used in newborns and compare the prediction of adequate serum levels based on doses calculated according to mg/kg/day and m2/day. This is an observational reprospective cohort at a referral neonatal unit, from 2011 to 2013. Newborns treated with vancomycin for the first episode of late-onset sepsis were included. Total dose in mg/kg/day, dose/m2/day, age, weight, body surface and...

Study on the susceptibility of Enterococcus faecalis from infectious processes to ciprofloxacin and vancomycin

Palavras-chave: Vancomycin; Drug dosage calculations; Pharmacokinetics; Infant; Newborn; Sepsis.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500451

J. Venom. Anim. Toxins incl. Trop. Dis.

Genaro,A.; Cunha,M. L. R. S.; Lopes,C. A. M..

Enterococcus faecalis is considered a pathogen responsible for hospital infections and, due to its frequent multi-resistant profile, has caused preoccupations among many medical authorities. The objective of this study was to determine the antimicrobial susceptibility of 74 strains isolated from blood cultures and purulent secretions to vancomycin and ciprofloxacin through the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by using the Microdilution test. The results showed a greater efficacy of vancomycin compared to ciprofloxacin (98.6% of the strains were inhibited by vancomycin at lower concentrations: 0.06 - 1 µg/ml). However, in the MBC analysis 73% of the strains showed a MBC of vancomycin only at high...

Successful treatment of vancomycin-resistant enterococcus ventriculitis in a child

Palavras-chave: Enterococcus faecalis; Vancomycin; Ciprofloxacin; Resistance; Spontaneous mutation.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300004